Department of Kinesiology
Molecular
Systems
Lab
The
primary goal of Dr. Spangenburg's NIH-funded laboratory is to
understand the molecular and cellular mechanisms that regulate adipose
tissue and skeletal
muscle
function. Dr. Spangenburg's lab utilizes an intergrative approach
to address these questions, which includes the use of cell culture,
animal models, and human studies. Ultimately, the goal of the
laboratory is to understand how physical activity and various
hormones/growth factors interact to enhance human function.
Members:
Director
Espen
Spangenburg, Ph. D.
301-405-2483 (office)
espen@umd.edu
Post-Doctoral Fellows
Sarah
Witkowski, Ph. D. (switkows@umd.edu)
Graduate Students
Lindsay
Wohlers (lwohlers@umd.edu)
Kathryn
Campbell (kacampbe@umd.edu)
Undergraduate Students
Alyssa Keating (alyssakeating@umd.edu)
Candace Receno (creceno@umd.edu)
Lab Alumni (Under construction)
Current Projects
The effect of
ovariectomy on
muscle and adipose tissue function. Loss of ovarian
function in female mammals results in the
accumulation of excess body fat, however the cellular mechanisms that
contribute
to this change in fat mass remain unknown. We are currently
investigating the role of ovarian hormones in the
maintenace of the adipose tissue function and skeletal muscle oxdative
phenotype. Further, we are trying to determine whether or not
physical activity is an effective substitute for HRT in postmenopausal
women. Specifically, we are trying to understand the interaction
of physical activity and ovarian hormones on cellular signaling
function in adipose tissue and skeletal muscle. We currently use
an intergrative scientific approach that consists of experiments in
cell culture, animal, and human models.
Figure 1. Oil-Red-O staining
of
Figure 2. Oil-Red-O
staining of skeletal muscle from
Figure 3.
ATGL protein expression in visceral
C2C12 myocytes after exposure
to SHAM and
OVX mice.
adipose tissue.
palmitate.
Equipment
The laboratory has a CO2 cell culture incubator,
inverted microscope, laminar flow hood. The lab also contains equipment
for
agarose and polyacrylamide gels, UV light box, power supply for
electrophoresis, high speed centrifuges, pH meter, Kodak Gel
Documentation
System, Real Time PCR (ABI 3100), SynGene GeneGnome (ECL quantification
system),
Columbus Instruments 6 lane mouse treadmill, 40 electronically
controlled
Layfayette Mouse Running Wheels, stirrers and shakers, temperature
controlled
water bath, shaking incubator, luminometer, balance, Beckman
UV/spectrophotometer, fume hood, -80º
and -20º C freezers, Eppendorf temperature controlled
microcentrifuge,
Eppendorf thermocycler, liquid N2 storage tank, and numerous other
items necessary for molecular and biochemical experiments (i.e.
pipetters,
glassware, vortexers, etc..).
Collaborators
Chris W.
Ward, Ph. D. School of Nursing, University of
Maryland-Baltimore
Debbie
M. Muoio, Ph. D. and Tim Koves, Ph. D., Duke University
Richard
Lovering, Ph. D. Dept of Physiology, School of Medicine,
University of Maryland-Baltimore
Stephen M. Roth,
Ph. D. Dept. Kinesiology, University of
Maryland-College Park
James
Hagberg, Ph. D. Dept Kinesiology, University of Maryland-College
Park
Dawn
Lowe, Ph. D. Program in Physical Therapy, University of Minnesota
Rosemary Schuh, Ph. D. Dept Neurology, University of Maryland-Baltimore
Todd McBride, Ph. D. California State University-Bakersfield
Representive List of Publications (out of 41 total)
Booth, F. W., M. V.
Chakravarthy, S. E.
Gordon, and E.
E. Spangenburg. Waging war on
physical inactivity: Using modern ammunition against an ancient enemy. Invited review. J
Appl. Physiol. (2002) 93: 3-30.
Booth, F. W., M. V.
Chakravarthy, and E.
E.
Spangenburg. Exercise and gene
expression: Physiological regulation of the human genome through
physical
activity. Invited review.
J Physiol.(London).
(2002) 543(2): 399-411.
E. E. Spangenburg, and F. W.
Booth. Molecular regulation of skeletal
muscle fiber type expression. Invited
Review. Acta. Physiol. Scand. (2003) 178: 413-424.
Rennie, M. J., H.
Wackerhage, E. E.
Spangenburg, and F. W.
Booth.
Control of human skeletal muscle mass.
Invited Review. Ann. Rev. Physiol. (2004) 66: 799-828.
Spangenburg, E. E. Suppressor of
cytokine
signaling-3 induces myoblast differentiation.
J Biol Chem. (2005)
280
(11): 10749–10758.
Spangenburg, E. E., D. A. Brown, M. S. Johnson, and R. L.
Moore. Exercise-induced increases in
SOCS-3
expression is associated with increases in IL-6 expression in muscle. J
Physiol. (2006) 572: 839-848.
Spangenburg,
E. E. and T. A. McBride. Inhibition
of stretch-activated channels during eccentric muscle contraction
attenuates
p70s6k activation. J
Appl Physiol. (2006) 100(1):129-35.
Sitnick,
M., A. Foley, M. B. Brown, and E. E. Spangenburg.
Ovariectomy prevents the recovery of
atrophied gastrocnemius skeletal muscle mass.
J Appl Physiol. (2006)
100(1):286-93. Received editorial
commentary.
Spangenburg, E. E. SOCS,
skeletal muscle, and chronic health conditions: the potential
interactions. Invited Review.
Exer
Sci Sport Rev. 2007
35(3):156-62.
Spangenburg,
E. E., D. LeRoith, C. W.
Ward, S. C.
Bodine. A functional IGF-I receptor is
not necessary for skeletal muscle hypertrophy.
J. Physiol. J. Physiol. (2008)
586(1): 283-291. Received
editorial commentary.
Wohlers, L. M., S. M.
Sweeney, R. M. Lovering, C. W. Ward, E. E.
Spangenburg. Changes in
contraction-induced
phosphorylation of AMP-activated protein kinase and mitogen activated
protein
kinases in skeletal muscle after ovariectomy.
J. Cell Biochem. (2009) 107: 171-178.
Booth,
F. W., M. J. Laye, and E. E. Spangenburg. Gold
standards for scientists who are
conducting animal-based exercise studies.
Viewpoint. J. Appl Physiol. In press.
Jenkins, N. T., S.
Witkowski, E. E. Spangenburg, J. M. Hagberg.
Effects of acute and chronic exercise on
intracellular nitric oxide in putative endothelial progenitor cells:
role of
NADPH oxidase. Am. J. Physiol. In
press.